iga detection  (Bio-Rad)

Journal: eBioMedicine

Article Title: A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaques

doi: 10.1016/j.ebiom.2025.105698

Figure Lengend Snippet: repNP is immunogenic in cynomolgus macaques. Groups of cynomolgus macaques were sham-vaccinated or vaccinated with repNP-alone or repNP + repGc (a). CCHFV-specific IgG to whole-virus antigen (b) or recombinant antigen (c) was quantified by ELISA. CCHFV-specific IFNγ responses at day −14 to overlapping peptide pools spanning the CCHFV GPC (G1-14) or NP (N1-5) was measured by ELISpot (d). The isotype of CCHFV NP-specific antibody was measured (e) and the ability of NP-specific antibody to bind Fc-receptors, TRIM21 or complement component C1q measured (f). Line connects means (b,d,e,f). (c) Data presented as mean and SD.

Article Snippet: The bound antigen-specific antibodies were subsequently probed with antibodies detecting baboon-IgG1, IgG2, IgG3, IgG4 (Absolute Antibody: Ab01619-3.0, Ab01620-23.0, Ab01621-1.1, Ab01622-3.0); and monkey-IgA (Fisher Scientific PIMA516729).

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Journal: eBioMedicine

Article Title: A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaques

doi: 10.1016/j.ebiom.2025.105698

Figure Lengend Snippet: Vaccinated animals develop anamnestic antibody responses to the NP and Gc. (a) IgG to recombinant NP, Gn and Gc was measured by ELISA at indicated timepoints relative to CCHFV challenge. (b) NP specific antibody was evaluated for its isotype and ability to bind Fc-receptors, C1q or TRIM21. Day 0 data is duplicated from Fig. 1 for comparison. (c) An IFNγ ELISpot was used to quantify anamnestic IFNγ responses to CCHFV peptides in PBMCs collected from animals at day 6 PI. (b,c) Line indicates mean.

Article Snippet: The bound antigen-specific antibodies were subsequently probed with antibodies detecting baboon-IgG1, IgG2, IgG3, IgG4 (Absolute Antibody: Ab01619-3.0, Ab01620-23.0, Ab01621-1.1, Ab01622-3.0); and monkey-IgA (Fisher Scientific PIMA516729).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Comparison, Enzyme-linked Immunospot

Journal: eBioMedicine

Article Title: A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaques

doi: 10.1016/j.ebiom.2025.105698

Figure Lengend Snippet: Single immunization with repNP is partially protective against CCHFV challenge. Groups of cynomolgus macaques were sham-vaccinated or vaccinated with repNP-alone (a). (b) CCHFV-specific IgG to whole-virus antigen was quantified by ELISA at indicated timepoint. On day 23 after vaccination, animals were challenged with CCHFV and animals were comprehensively scored daily for signs of clinical disease (c) and AST (d), ALT (e) and platelet levels in the blood enumerated (f). Viral loads in the blood (g) or various tissues collected on day 5 or 6 PI (h) were quantified by qRT-PCR. Formalin fixed tissue sections were stained for CCHFV NP antigen (i) or with H&E (j) and scored by pathologists blinded to study group for presence of viral antigen or lesions. (h–j) Data shown as mean and SD. (b) Line indicates mean.

Article Snippet: The bound antigen-specific antibodies were subsequently probed with antibodies detecting baboon-IgG1, IgG2, IgG3, IgG4 (Absolute Antibody: Ab01619-3.0, Ab01620-23.0, Ab01621-1.1, Ab01622-3.0); and monkey-IgA (Fisher Scientific PIMA516729).

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Staining